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Equine Health

Equine Health - Reading Blood Tests

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If your horse requires blood tests to help diagnose his/her condition, here are some of the most common counters noted on test result reports.  Blood tests are highly useful for checking on the true health of your horse. 

You can request a blood test from your vet at any time to check his\her biochemistry.  Doing this on a regular basis, perhaps once a year with vaccinations or other scheduled visits, is an excellent way to keep a check that you aren't under or over supplementing before it becomes a severe problem.

Your vet may supply you with the printout of your horse's results as well as telling you what they mean, the following are usually on the printout:

  • RBC   Red Blood Cells, indicates the oxygen carrying capacity of the blood.

    • Haemoglobin: Most laboratories measure haemoglobin the same way and the range for optimum oxygen capacity is 14.5 - 15.5 gm/100 ml of blood. Levels below 12.5 and above 16.0 will affect performance. The lower reading indicates an anaemic state and the higher reading can indicate dehydration.

     

  • PCV Packed Cell Volume:

    • This is mostly the percentage of cells within the blood. The red cells have the larger percentage as there are usually 6-8 million/ml as against white cells of 6-8 thousand/ml.

    • PCV is used to assess dehydration and anaemia.

    • Optimum levels are close to 40% with levels below 35% and above 45% likely to indicate problems.

    • This reading is also affected by excitement at collection.

    • PCV also rises as fitness increases, often though, it keeps rising when problems occur because dehydration is a common sequel of work-related problems.

    • Generally a horse is considered anaemic if the packed cell volume (PCV) is less than 35% or less than 2 standard deviation units below that horse's average count.

    • If you are concerned your horse is anaemic then you are best to collect blood within a few minutes of completing 800-1000 metres of three-quarter pace work.

  • Mean Corpuscular Volume: MCV is the size of the red blood cell and is important in two fields. One relates to anaemia cause diagnosis.

    • If the figure is high the horse has anaemia, the cause is usually blood loss (ulcers, bleeder), Vitamin B6, B12, Folic Acid or niacin deficiencies or gut upsets causing reduced production of these vitamins.

    • If the figure is low with anaemia, this usually indicates iron deficiency but also copper or pyridoxine.

     

  • Mean Corpuscular Haemoglobin Concentration: MCHC is a measure of the amount of haemoglobin in each red blood cell.

    • Usually this is in a tight range of 35- 39%.

    • It cannot be above 40% so any reading with a figure greater than 40% is wrong.

    • Variations in MCHC usually reflect problems in the other readings as most labs calculate MCHC by dividing the haemoglobin by PCV and multiplying by 100.

     

  • WBC   White Blood Cells, a high WBC means that an infection has been detected and white blood cells are being created to fight the infection.

  • Proteins   6 proteins are usually listed, raised globulin and fibrinogen levels are signs of infection.

  • Biochemistry   will show up mineral deficiencies and shows enzyme levels.  High levels of enzymes can indicate cell damage (which causes enzymes to leak into the blood stream).  The biochemistry section reveals much about the nutrition of your horse - no matter how much you feed your horse, if it is deficient or overdosed in one or two key vitamins or minerals it cannot make good use of the nutrition you provide. 

It is important to recognise that because the bulk of your horse's nutrition comes from forage (whether pasture or hay) you are then reliant on the soil that this is grown on for the minerals it contains.  New Zealand (and many parts of North America) soils are frequently deficient in Selenium, other parts of the world have their own mineral characteristics and these are reflected in the forage they produce.  The only way to gauge what and how much to supplement is either to have a complete analysis of all feeds (incuding each batch of forage) or to have your horse blood tested.  This is particularly important with may minerals as they often have a very narrow threshold of tolerance, over supplementing is just as dangerous as under supply.

  • "Gram's Stains" are simply an analysis of the types and quantities of organisms e.g. bacteria that are present in a sample of blood or fluid.  The sample is sent off to a laboratory for analysis and are cultured to identify the organisms present.  Effective treatment by antibiotics or other drugs can only be achieved if the drug is correct for the type of organism.
  • Gram Negative Bacilli - This describes several groups of organisms rather than a lack of bacteria which the name seems to imply.
  • Leucocytes (leukocytes) Test results may refer to the presence of or provide a count of leucocytes and/or other cells of the lymphoid system.  These cells which originate in the bone marrow or liver have various roles in fighting infection.  There are four major cell types'; erythrocytes,platelets (promote blood clotting and inflammation), granulocytes and monocytes/macrophages and lymphocytes and natural killers.
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First Aid When reading your horse's blood test results you (and your vet) should also consider the following factors.
  • The only thing that causes haemoglobin to rise is work. All that additives do is provide the raw materials to make haemoglobin, no work means no rise in haemoglobin. Horses in the paddock do perfectly well on 10.5 - 12.5 and when they return to the stables, these are normal readings.  The aim of training is to stress the system so the bone marrow makes more haemoglobin for the workload, thus, a gradual increasing workload is best.
  • Young horses will usually achieve adult iron levels by the time they are yearlings and the PCV of two year olds in training will usually increase by about 4% per month for the first 5 months, after which it will generally remain stable.
  • Measuring the red blood cell count is generally considered to be paramount. However, a horse at rest will have 50% of his red blood cells lying in wait within his spleen. These red cells are then released under the effect of adrenalin when excited or exercising. So, while measuring red cell counts at rest is useful it doesn't tell you the total number of red cells available during exercise.
  • There can be up to 20% variation in a horse's red cell count over a single week.
  • Post exercise bloods may be more reliable and some studies have shown a relationship between higher haemoglobin levels and performance. Ideally a fit healthy horse should have a PCV of 60-65% and Hb 200-230 of gm/L after exercise.
  • Once collected blood should be analysed within 6 hours to prevent abnormal results. Storing blood for greater than 12 hours will result in lower red cell counts, lower glucose levels and abnormal levels of muscle enzymes (AST) and electrolytes.
 
 

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Virtually Horses 01 Dec 2001